Zinc Regulates Chemical-Transmitter Storage in Nanometer Vesicles and Exocytosis Dynamics as Measured by Amperometry
Artikel i vetenskaplig tidskrift, 2017
We applied electrochemical techniques with nano-tip electrodes to show that micromolar concentrations of zinc not only trigger changes in the dynamics of exocytosis, but also vesicle content in a model cell line. The vesicle catecholamine content in PC12 cells is significantly decreased after 100 mm zinc treatment, but, catecholamine release during exocytosis remains nearly the same. This contrasts with the number of molecules stored in the exocytosis vesicles, which decreases, and we find that the amount of catecholamine released from zinc-treated cells reaches nearly 100% content expelled. Further investigation shows that zinc slows down exocytotic release. Our results provide the missing link between zinc and the regulation of neurotransmitter release processes, which might be important in memory formation and storage.
Chromaffin
vesicle content
Extended Kiss
exocytosis
Protein-Kinase-C
PC12 Cells
amperometry
Release
Fusion Pore
Cells
Quantal Size
zinc
Kiss-and-Run
In-Vitro
Actin
cytometry
Författare
Lin Ren
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Masoumeh Dowlatshahi Pour
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Soodabeh Majdi
Göteborgs universitet
Li Xianchan
Göteborgs universitet
Per Malmberg
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Andrew Ewing
Chalmers, Kemi och kemiteknik, Kemi och biokemi
Angewandte Chemie - International Edition
1433-7851 (ISSN) 1521-3773 (eISSN)
Vol. 56 18 4970-4975Ämneskategorier
Oorganisk kemi
DOI
10.1002/anie.201700095