A glucuronoyl esterase from Acremonium alcalophilum cleaves native lignin-carbohydrate ester bonds
Artikel i vetenskaplig tidskrift, 2016
The Glucuronoyl esterases (GE) have been proposed to target lignin-carbohydrate (LC) ester bonds between lignin moieties and glucuronic acid side groups of xylan, but to date, no direct observations of enzymatic cleavage on native LC ester bonds have been demonstrated. In the present investigation, LCC fractions from spruce and birch were treated with a recombinantly produced GE originating from Acremonium alcalophilum (AaGE1). A combination of size exclusion chromatography and 31P NMR analyses of phosphitylated LCC samples, before and after AaGE1 treatment provided the first evidence for cleavage of the LC ester linkages existing in wood.
Författare
Jenny Arnling Bååth
Wallenberg Wood Science Center (WWSC)
Chalmers, Biologi och bioteknik, Industriell bioteknik
Nicola Giummarella
Kungliga Tekniska Högskolan (KTH)
Sylvia Klaubauf
Chalmers, Biologi och bioteknik, Industriell bioteknik
Martin Lawoko
Kungliga Tekniska Högskolan (KTH)
Lisbeth Olsson
Wallenberg Wood Science Center (WWSC)
Chalmers, Biologi och bioteknik, Industriell bioteknik
FEBS Letters
0014-5793 (ISSN) 18733468 (eISSN)
Vol. 590 16 2611-8Ämneskategorier
Biokemi och molekylärbiologi
Analytisk kemi
Styrkeområden
Livsvetenskaper och teknik (2010-2018)
DOI
10.1002/1873-3468.12290