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Supercoil-Accelerated DNA Threading Intercalation

Pär Nordell (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Erik T. Jansson (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; Per Lincoln (Institutionen för kemi- och bioteknik, Fysikalisk kemi)
Biochemistry (0006-2960). Vol. 48 (2009), 7, p. 1442-1444.
[Artikel, refereegranskad vetenskaplig]

The effect of DNA supercoiling on a sterically very demanding threading intercalation process is here investigated. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is two orders of magnitude higher than into the cleaved linear form. Further saturation is however, in comparison to the linear DNA, kinetically hampered. We also observe that threading kinetics correlates with the inhibition of luciferase expression from the plasmid construct. The results show how the target torsional strain can function as control of DNA threading kinetics and gene expression efficiency.



Denna post skapades 2009-09-16. Senast ändrad 2013-10-29.
CPL Pubid: 98157

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)

Ämnesområden

Fysikalisk kemi
Biokemi

Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:


Kinetic Recognition of Nucleic Acids - Studies on the DNA Binding Selectivity of Threading Ruthenium Complexes