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The sodium pump Ena1p provides mechanistic insight into the salt sensitivity of vacuolar protein sorting mutants

Katarina Logg (Institutionen för teknisk fysik, Bionanofotonik) ; Jonas Warringer ; Sayed Hossein Hashemi ; Mikael Käll (Institutionen för teknisk fysik, Bionanofotonik) ; Anders Blomberg
BBA - Molecular Cell Research (0167-4889). Vol. 1783 (2008), 6, p. 974-984.
[Artikel, refereegranskad vetenskaplig]

The vacuolar/endosomal network has an important but as yet undefined role in the cellular tolerance to salt stress. We hypothesized that the mechanistic basis for the importance of vacuolar protein sorting (vps) components in salt tolerance is the targeting of the crucial sodium exporter Ena1p to the plasma membrane. The link between Ena1p and the vps components was established by the observation that overexpression of Ena1p could suppress the salt sensitivity of the ESCRT knockouts vps20Δ, snf7/vps32Δ and snf8/vps22Δ. To further investigate this functional interaction, fluorescence microscopy was utilized to monitor localization of GFP-tagged Ena1p. For all analyzed vps mutants, Ena1p seemed properly localized to the plasma membrane, even during saline growth. However, quantitative differences in plasma membrane localized Ena1p were recorded; e.g. the highly salt sensitive pep12Δ mutant exhibited substantially enhanced Ena1p levels. In addition, the kinetics of Ena1p localization to the plasma membrane was severely delayed in several vps mutants, and this delay correlated to the salt specific growth defect. This paper discusses potential mechanistic hypotheses, like Ena1p transporter activity or localization kinetics, or ESCRT component's influence on signaling, for linking endosomal sorting functions to cellular salt sensitivity.

Nyckelord: vacuolar protein sorting, sodium export , yeast , GFP, vps deletion mutant, phenotype



Denna post skapades 2009-03-05. Senast ändrad 2016-08-19.
CPL Pubid: 91099

 

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Institutioner (Chalmers)

Institutionen för teknisk fysik, Bionanofotonik (2007-2015)
Institutionen för cell- och molekylärbiologi (1994-2011)

Ämnesområden

Optik
Cell- och molekylärbiologi

Chalmers infrastruktur

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