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Blood group antigen recognition by Escherichia coli heat-labile enterotoxin

Åsa Holmner (Institutionen för kemi- och bioteknik) ; G. Askarieh ; M. Okvist ; Ute Krengel (Institutionen för kemi- och bioteknik, Molekylär bioteknik)
Journal of Molecular Biology (0022-2836). Vol. 371 (2007), 3, p. 754-764.
[Artikel, refereegranskad vetenskaplig]

In a number of bacterial infections, such as Helicobacter pylori, Campylobacter jejuni and Vibrio cholerae infections, a correlation between the severity of disease and blood group phenotype of infected individuals has been observed. In the present investigation, we have studied the molecular basis of this effect for enterotoxigenic Escherichia coli (ETEC) infections. ETEC are non-invasive bacteria, which act through second messenger pathways to cause diarrhea. It has been suggested that the major virulence factor of ETEC from human isolates, i.e. the human heat-labile enterotoxin (hLT), recognizes certain blood group epitopes, although the molecular basis of blood group antigen recognition is unknown. The 2.5 angstrom crystal structure of the receptor-binding B-subunit of hLT in complex with the blood group A antigen analog GalNAc alpha 3(Fuc alpha-2)Gal beta 4(Fuc alpha-3)Glc beta provides evidence of a previously unknown binding site in the native toxin. The structure reveals the molecular interactions underlying blood group antigen recognition and suggests how this protein can discriminate between different blood group epitopes. These results support the previously debated role of hLT in the blood group dependence of ETEC infections. Similar observations regarding the closely related cholera toxin in V. cholera infections are also discussed. (c) 2007 Elsevier Ltd. All rights reserved.

Nyckelord: blood-group recognition, bacterial toxins, clinical/epidemiological, data, protein-carbohydrate interactions, X-ray crystal structure, SURFACE-PLASMON RESONANCE, PIG INTESTINAL-MUCOSA, CHOLERA-TOXIN, BINDING, GROUP-A, CRYSTAL-STRUCTURE, EPITHELIAL-CELLS, VIBRIO-CHOLERAE, PROTEIN-STRUCTURE, DIFFRACTION DATA, GROUP SYSTEM



Denna post skapades 2008-12-11. Senast ändrad 2012-01-18.
CPL Pubid: 81111

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik (2005-2014)
Institutionen för kemi- och bioteknik, Molekylär bioteknik (2005-2007)

Ämnesområden

Kemiteknik

Chalmers infrastruktur