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Physiological and morphological study of encapsulated Saccharomyces cerevisiae

Farid Talebnia ; Mohammad J. Taherzadeh (Institutionen för kemi- och bioteknik)
Enzyme and Microbial Technology (0141-0229). Vol. 41 (2007), 6-7, p. 683-688.
[Artikel, refereegranskad vetenskaplig]

The impact of encapsulation on the anaerobic growth pattern of S. cerevisiae CBS 8066 in a defined synthetic medium over 20 consecutive batch cultivations was investigated. In this period, the ethanol yield increased from 0.43 to 0.46 g/g, while the biomass and glycerol yields decreased by 5 8 and 23%, respectively. The growth rate of the encapsulated cells in the first batch was 0.13 h(-1), but decreased gradually to 0.01 h(-1) within the 20 sequential batch cultivations. Total RNA content of these yeast cells decreased by 39% from 90.3 to 55 mg/g, while the total protein content decreased by 24% from 460 to 350 mg/g. On the other hand, the stored carbohydrates, that is, glycogen and trehalose content, increased by factors of 4.5 and 4 within 20 batch cultivations, respectively. Higher biomass concentrations inside capsules led to a lower glucose diffusion rate through the membrane, and volumetric mass transfer coefficient for glucose was drastically decreased from 6.28 to 1.24 (cm(3)/min) by continuing the experiments. Most of the encapsulated yeast existed in the form of single and non-budding cells after long-term application. (C) 2007 Elsevier Inc. All rights reserved.

Nyckelord: encapsulation, yeast physiology, ethanol, glycogen, proteins, mass, transfer coefficient, CONTINUOUS CULTIVATION, ETHANOL-PRODUCTION, ACID HYDROLYZATE, GROWTH-RATE, FERMENTATION, YEAST, IMMOBILIZATION, GLUCOSE, CELLS, PERFORMANCE

Denna post skapades 2008-12-10. Senast ändrad 2010-09-13.
CPL Pubid: 80917


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Institutionen för kemi- och bioteknik (2005-2014)



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