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Towards a biosensor immunoassay of protein-bound isopeptides in human plasma

Anna Bergstrand (Institutionen för kemi- och bioteknik, Teknisk ytkemi) ; Ismail Ceylan ; Gerard Quash ; Magnus Nydén (Institutionen för kemi- och bioteknik, Teknisk ytkemi ; SuMo Biomaterials) ; Krister Holmberg (Institutionen för kemi- och bioteknik, Teknisk ytkemi)
Colloids and Surfaces B: Biointerfaces (0927-7765). Vol. 66 (2008), p. 150.
[Artikel, refereegranskad vetenskaplig]

This study demonstrates that synthetic isopeptides formed on BSA can be quantitatively analyzed by a surface plasmon resonance-based biosensor method. A monoclonal IgM antibody 81D4, that reacts with the synthetic isopeptide and also with the natural isopeptide cross-link in D-dimer (but not with its non-cross-linked fibrin monomer), was covalently immobilized to a carboxymethylated dextran surface, a CM5 surface. Its immunocapturing efficiency was found to be good. The affinity of the interaction between the monoclonal 81D4 and the synthetic isopeptide was estimated to approximately 4x10(-7)M. Good reactivity was also observed when human plasma spiked with this isopeptide was used as test solution. Cross-linked D-dimer in the plasma of patients is a marker of disseminated intravascular coagulation (DIC) which occurs late in sepsis. This biosensor method has the potential to be developed into a rapid sensitive assay for measuring the level of natural isopeptide cross-links in proteins in the plasma of patients with a suspected diagnosis of sepsis.

Denna post skapades 2008-12-10. Senast ändrad 2017-06-29.
CPL Pubid: 80872


Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Teknisk ytkemi (2005-2014)
SuMo Biomaterials



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