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Fed-batch cultivation of Mucor indicus in dilute-acid lignocellulosic hydrolyzate for ethanol production

Keikhosro Karimi ; Tomas Brandberg (Institutionen för kemi- och bioteknik, Kemisk reaktionsteknik) ; Lars Edebo ; Mohammad J. Taherzadeh
BIOTECHNOLOGY LETTERS (0141-5492). Vol. 27 (2005), 18, p. 1395-U1.
[Artikel, refereegranskad vetenskaplig]

Mucor indicus fermented dilute-acid lignocellulosic hydrolyzates to ethanol in fed-batch cultivation with complete hexose utilization and partial uptake of xylose. The fungus was tolerant to the inhibitors present in the hydrolyzates. It grew in media containing furfural (1 g/l), hydroxymethylfurfural (1 g/l), vanillin (1 g/l), or acetic acid (7 g/l), but did not germinate directly in the hydrolyzate. However, with fed-batch methodology, after initial growth of M. indicus in 500 ml enzymatic wheat hydrolyzate, lignocellulosic hydrolyzate was fermented with feeding rates 55 and 100 ml/h. The fungus consumed more than 46% of the initial xylose, while less than half of this xylose was excreted in the form of xylitol. The ethanol yield was 0.43 g/g total consumed sugar, and reached the maximum concentration of 19.6 g ethanol/l at the end of feeding phase. Filamentous growth, which is regarded as the main obstacle to large-scale cultivation of M. indicus, was avoided in the fed-batch experiments.

Denna post skapades 2008-12-09. Senast ändrad 2010-01-26.
CPL Pubid: 80539


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Kemisk reaktionsteknik (2005-2014)
Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi (1991-2005)


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