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A Lipidic-Sponge Phase Screen for Membrane Protein Crystallization

Annemarie Wöhri (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Linda C Johansson ; Pia Wadsten-Hindrichsen (Institutionen för kemi- och bioteknik, Farmaceutisk teknologi) ; Weixiao Yuan Wahlgren ; Gerhard Fischer ; Rob Horsefield ; Gergely Katona ; Maria Nyblom (Institutionen för kemi- och bioteknik, Molekylär mikroskopi) ; Fredrik Öberg ; G Young ; Richard Cogdell ; N J Fraser ; Sven Engström (Institutionen för kemi- och bioteknik, Teknisk ytkemi) ; Richard Neutze
Structure (0969-2126). Vol. 16 (2008), 7, p. 1003-1009.
[Artikel, refereegranskad vetenskaplig]

A major current deficit in structural biology is the lack of high-resolution structures of eukaryotic membrane proteins, many of which are key drug targets for the treatment of disease. Numerous eukaryotic membrane proteins require specific lipids for their stability and activity, and efforts to crystallize and solve the structures of membrane proteins that do not address the issue of lipids frequently end in failure rather than success. To help address this problem, we have developed a sparse matrix crystallization screen consisting of 48 lipidic-sponge phase conditions. Sponge phases form liquid lipid bilayer environments which are suitable for conventional hanging- and sitting-drop crystallization experiments. Using the sponge phase screen, we obtained crystals of several different membrane proteins from bacterial and eukaryotic sources. We also demonstrate how the screen may be manipulated by incorporating specific lipids such as cholesterol; this modification led to crystals being recovered from a bacterial photosynthetic core complex.



Denna post skapades 2008-07-09. Senast ändrad 2016-08-17.
CPL Pubid: 72384

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär mikroskopi (2008-2014)
Institutionen för kemi (2001-2011)
Institutionen för kemi- och bioteknik, Farmaceutisk teknologi (2005-2014)
Institutionen för kemi- och bioteknik, Teknisk ytkemi (2005-2014)

Ämnesområden

Kemi
Cell- och molekylärbiologi

Chalmers infrastruktur

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Lipidic-sponge phase crystallisation and time-resolved Laue diffraction studies of bacterial photosynthetic reaction centres