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Dual-CARS microscopy

Annika Enejder (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; Christian Brackmann (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; Ondrej Burkacky ; Madeleine Åkeson (Institutionen för kemi- och bioteknik, Molekylär bioteknik)
PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE) (0277-786X ). Vol. 6442 (2007), 15,
[Konferensbidrag, refereegranskat]

We present a new Coherent Anti-Stokes Raman Scattering (CARS) microscopy technique for label-free imaging of bio-molecules in living cells; dual-CARS microscopy. The use of three synchronized laser pulses in a dual-pump/dual-detection configuration enables imaging of two species with different molecular vibrations simultaneously, as well as acquisition of images free of non-resonant background. We show the power of the method by imaging deuterated nonadecane slowly diffusing into a suspension of living yeast cells in medium, clearly distinguishing the medium and the lipid droplets in the cells by probing the CH2 vibration from the D-nonadecane by probing the CD vibration. In addition, images of lipid stores in living C. elegans nematodes free of non-resonant background are shown. This results in a significant enhancement of the image contrast, allowing the visualization of emerging, low-density lipid stores in a dauer larva, difficult to distinguish in conventional CARS microscopy. The separation of the non-resonant background is shown to be beneficial also when monitoring molecules with weak vibrational modes. The improved sensitivity obtained is illustrated by probing the C=C vibration in polyunsaturated lipids extracted from fish. This enables the monitoring of the degree of unsaturation of lipids, a high value of which is reported in foods known to have positive effects on human health.

Nyckelord: non-linear microscopy; CARS; vibrational imaging; chemical imaging; image contrast enhancement; unsaturated lipids; Caenorhabditis elegans; Saccharomyces cerevisiae


Conference on Multiphoton Microscopy in the Biomedical Sciences VII San Jose, CA, JAN 21-23, 2007



Denna post skapades 2008-01-14. Senast ändrad 2017-01-27.
CPL Pubid: 66500

 

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär bioteknik (2005-2007)

Ämnesområden

Optisk fysik

Chalmers infrastruktur