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Simultaneous maximization of cell permeabilization and viability in single-cell electroporation using an electrolyte-filled capillary

A. Agarwal ; I. Zudans ; Owe Orwar (Institutionen för kemi- och bioteknik, Fysikalisk kemi) ; S. G. Weber
Analytical Chemistry (0003-2700). Vol. 79 (2007), 1, p. 161-167.
[Artikel, refereegranskad vetenskaplig]

A549 cells were briefly exposed to Thioglo-1, which converts thiols to fluorescent adducts. The fluorescent cells were exposed to short (50-300 ms) electric field pulses (500 V across a 15 cm capillary) created at the tip of an electrolyte-filled capillary. Fluorescence microscopy revealed varying degrees of cell permeabilization depending on the conditions. Longer pulses and a shorter cell-capillary tip distance led to a greater decrease in the cell's fluorescence. Live/dead (calcein AM and propidium iodide) testing revealed that a certain fraction of cells died. Longer pulses and shorter cell-capillary tip distances were more deadly. An optimum condition exists at a cell-capillary tip distance of 3.5-4.5 mu m and a pulse duration of 120-150 ms. At these conditions, > 90% of the cells are permeabilized and 80-90% survive.

Denna post skapades 2008-01-08. Senast ändrad 2008-09-18.
CPL Pubid: 64887


Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Fysikalisk kemi (2005-2014)



Chalmers infrastruktur