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Taxonomic reliability of DNA sequences in public sequence databases: A fungal perspective

R. Henrik Nilsson ; Martin Ryberg ; Erik Kristiansson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Kessy Abarenkov ; Karl-Henrik Larsson ; Urmas Kõljalg
PLoS ONE Vol. 1 (2006), 1, p. e59.
[Artikel, refereegranskad vetenskaplig]

Background DNA sequences are increasingly seen as one of the primary information sources for species identification in many organism groups. Such approaches, popularly known as barcoding, are underpinned by the assumption that the reference databases used for comparison are sufficiently complete and feature correctly and informatively annotated entries. Methodology/Principal Findings The present study uses a large set of fungal DNA sequences from the inclusive International Nucleotide Sequence Database to show that the taxon sampling of fungi is far from complete, that about 20% of the entries may be incorrectly identified to species level, and that the majority of entries lack descriptive and up-to-date annotations. Conclusions The problems with taxonomic reliability and insufficient annotations in public DNA repositories form a tangible obstacle to sequence-based species identification, and it is manifest that the greatest challenges to biological barcoding will be of taxonomical, rather than technical, nature.



Denna post skapades 2007-10-31. Senast ändrad 2011-06-29.
CPL Pubid: 60157

 

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Institutioner (Chalmers)

Institutionen för växt- och miljövetenskaper (2006-2011)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)

Ämnesområden

Biologiska vetenskaper

Chalmers infrastruktur