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Evaluation of reference genes for studies of gene expression in human adipose tissue.

Britt G. Gabrielsson ; Louise Olofsson ; Anders Sjögren (Institutionen för matematiska vetenskaper, matematisk statistik) ; Margareta Jernås ; Anna Elander ; Malin Lönn ; Mats Rudemo (Institutionen för matematiska vetenskaper, matematisk statistik) ; Lena M S Carlsson
Obesity research (1071-7323). Vol. 13 (2005), 4, p. 649-52.
[Artikel, refereegranskad vetenskaplig]

OBJECTIVE: The aim of this study was to evaluate reference genes for expression studies of human adipose tissue. RESEARCH METHODS AND PROCEDURES: Using 52 human adipose tissue expression profiles (HU95), 10 putative reference genes with the lowest variation in expression levels were selected for further studies. Expression stability of these 10 novel and 5 previously established reference genes was evaluated by real-time reverse transcriptase-polymerase chain reaction analysis. For this purpose, 44 adipose tissue biopsies from 27 subjects were chosen to include a wide range of parameters such as sex, age, BMI, depot origin, biopsy procedure, and effects of nutrition. RESULTS: LRP10 was identified as the gene with the least variation in expression levels. The frequently used reference genes RPLP0, 18S rRNA, PPIA, ACTB, and GAPD were ranked as 4, 6, 7, 8, and 10, respectively. DISCUSSION: Our results suggest that LRP10 is a better choice as reference for expression studies of human adipose tissue compared with the most frequently used reference genes.

Nyckelord: Adipose Tissue, metabolism, Adult, Aging, Biopsy, methods, Body Mass Index, Female, Gene Expression, Gene Expression Profiling, Humans, LDL-Receptor Related Proteins, Male, Membrane Transport Proteins, genetics, Middle Aged, Oligonucleotide Array Sequence Analysis, Receptors, LDL, genetics, Reverse Transcriptase Polymerase Chain Reaction, Sex Characteristics

Denna post skapades 2007-10-26. Senast ändrad 2012-06-21.
CPL Pubid: 58010


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Institutioner (Chalmers)

Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism (1991-2005)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi (1991-2005)


Medicinsk genetik

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