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Neural cell adhesion molecule-deficient beta-cell tumorigenesis results in diminished extracellular matrix molecule expression and tumour cell-matrix adhesion

Joakim Håkansson ; Xiaojie Xian ; Liqun He ; Anders Ståhlberg (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; Sven Nelander ; Tore Samuelsson ; Mikael Kubista (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; Henrik Semb
Tumour Biology (1010-4283). Vol. 26 (2005), 2, p. 103-112.
[Artikel, refereegranskad vetenskaplig]

To understand by which mechanism neural cell adhesion molecule (N-CAM) limits beta tumour cell disaggregation and dissemination, we searched for potential downstream genes of N-CAM during beta tumour cell progression by gene expression profiling. Here, we show that N-CAM-deficient beta-cell tumorigenesis is associated with changes in the expression of genes involved in cell-matrix adhesion and cytoskeletal dynamics, biological processes known to affect the invasive and metastatic behaviour of tumour cells. The extracellular matrix (ECM) molecules emerged as the primary target, i.e. N-CAM deficiency resulted in down-regulated mRNA expression of a broad range of ECM molecules. Consistent with this result, deficient deposition of major ECM stromal components, such as fibronectin, laminin 1 and collagen IV, was observed. Moreover, N-CAM-deficient tumour cells displayed defective matrix adhesion. These results offer a potential mechanism for tumour cell disaggregation during N-CAM-deficient beta tumour cell progression. Prospective consequences of these findings for the role of N-CAM in beta tumour cell dissemination are discussed.

Nyckelord: Animals, Cell Transformation, Neoplastic, Disease Progression, Extracellular Matrix Proteins, metabolism, Gene Expression Profiling, Insulinoma, metabolism, pathology, Mice, Mice, Inbred C57BL, Mice, Knockout, Neural Cell Adhesion Molecules, genetics, physiology, Oligonucleotide Array Sequence Analysis, RNA, Messenger, genetics, metabolism, Reverse Transcriptase Polymerase Chain Reaction, Tumor Markers, Biological, genetics, metabolism



Denna post skapades 2007-10-10. Senast ändrad 2013-07-18.
CPL Pubid: 52457

 

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Institutioner (Chalmers)

Institutionen för medicinsk och fysiologisk kemi (1991-2005)
Institutionen för kemi- och bioteknik, Molekylär bioteknik (2005-2007)

Ämnesområden

Molekylärbiologi
MEDICIN OCH HÄLSOVETENSKAP

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