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Imbalance of the mitochondrial pro- and anti-apoptotic mediators in neuroblastoma tumours with unfavourable biology.

Frida Abel ; Rose-Marie Sjöberg ; Staffan Nilsson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Per Kogner ; Tommy Martinsson
European journal of cancer (Oxford, England : 1990) (0959-8049). Vol. 41 (2005), 4, p. 635-46.
[Artikel, refereegranskad vetenskaplig]

It has been proposed that a lack of apoptosis plays an important role in neuroblastoma (NB) progression. We therefore screened cDNA array filters, including 198 apoptotic genes, in order to identify mRNA transcripts that are differentially expressed in tumours with unfavourable versus favourable biology. Twenty-one genes were analysed further using real-time reverse-transcriptase-polymerase chain reaction (RT-PCR). Significantly lower levels of DNCL1 (PIN; P(c)(corrected) = 0.0054) and NTRK1 (TrkA; P(c) = 0.039) were found in NB tumours with unfavourable biology. In addition, BID, BCL2, APAF1, CASP2, CASP3 and CASP9 were found to be preferentially expressed in tumours with favourable biology, whereas CDKN1A (p21), IL2RA, and MCL1, were found to be preferentially expressed in NB tumours with unfavourable biology. In conclusion, mRNA levels of transcripts encoding pro-apoptotic mediators of the mitochondrial apoptotic pathway were found to be expressed to a lower extent in tumours with unfavourable biology. Our data also suggest that the mitochondrial pathway is suppressed in advanced stages of NB tumours, due to an imbalance between anti-apoptotic and pro-apoptotic mediators which is a finding that may have therapeutic significance.

Nyckelord: Apoptosis, genetics, DNA, Complementary, genetics, Humans, Mitochondria, genetics, pathology, Neuroblastoma, genetics, pathology, RNA Precursors, genetics, RNA, Messenger, genetics, Reverse Transcriptase Polymerase Chain Reaction, methods



Denna post skapades 2007-10-09. Senast ändrad 2015-01-16.
CPL Pubid: 52309

 

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Institutioner (Chalmers)

Institutionen för kvinnors och barns hälsa, Avdelningen för pediatrik (1991-2005)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)

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MEDICIN OCH HÄLSOVETENSKAP

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