CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

The myxoid/round cell liposarcoma fusion oncogene FUS-DDIT3 and the normal DDIT3 induce a liposarcoma phenotype in transfected human fibrosarcoma cells.

Katarina Engström ; Helena Willén ; Christina Kåbjörn-Gustafsson ; Carola Andersson ; Marita Olsson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Melker Göransson ; Sofia Järnum ; Anita Olofsson ; Elisabet Warnhammar Finnborg ; Pierre Åman
The American journal of pathology (0002-9440). Vol. 168 (2006), 5, p. 1642-53.
[Artikel, refereegranskad vetenskaplig]

Myxoid/round cell liposarcoma (MLS/RCLS) is the most common subtype of liposarcoma. Most MLS/RCLS carry a t(12;16) translocation, resulting in a FUS-DDIT3 fusion gene. We investigated the role of the FUS-DDIT3 fusion in the development of MLS/RCLS in FUS-DDIT3- and DDIT3-transfected human HT1080 sarcoma cells. Cells expressing FUS-DDIT3 and DDIT3 grew as liposarcomas in severe combined immunodeficient mice and exhibited a capillary network morphology that was similar to networks of MLS/RCLS. Microarray-based comparison of HT1080, the transfected cells, and an MLS/RCLS-derived cell line showed that the FUS-DDIT3- and DDIT3-transfected variants shifted toward an MLS/RCLS-like expression pattern. DDIT3-transfected cells responded in vitro to adipogenic factors by accumulation of fat and transformation to a lipoblast-like morphology. In conclusion, because the fusion oncogene FUS-DDIT3 and the normal DDIT3 induce a liposarcoma phenotype when expressed in a primitive sarcoma cell line, MLS/RCLS may develop from cell types other than preadipocytes. This may explain the preferential occurrence of MLS/RCLS in nonadipose tissues. In addition, development of lipoblasts and the typical MLS/RCLS capillary network could be an effect of the DDIT3 transcription factor partner of the fusion oncogene.

Nyckelord: Adipogenesis, Animals, Cluster Analysis, Down-Regulation, Female, Fibrosarcoma, blood supply, metabolism, Gene Expression Regulation, Neoplastic, Humans, Liposarcoma, Myxoid, blood supply, metabolism, Mice, Mice, SCID, Oligonucleotide Array Sequence Analysis, Oncogene Proteins, Fusion, physiology, RNA-Binding Protein FUS, genetics, metabolism, physiology, Transcription Factor CHOP, metabolism, physiology, Transfection, Up-Regulation



Denna post skapades 2007-10-04. Senast ändrad 2011-01-20.
CPL Pubid: 51366

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kliniska vetenskaper, sektionen för onkologi, radiofysik, radiologi och urologi (GU)
Institutionen för biomedicin, avdelningen för patologi (GU)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)

Ämnesområden

Cell- och molekylärbiologi

Chalmers infrastruktur