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Glomerulus-specific mRNA transcripts and proteins identified through kidney expressed sequence tag database analysis

L He ; Y Sun ; Jaakko Patrakka ; Petter Mostad (Institutionen för matematiska vetenskaper, matematisk statistik) ; Jenny Norlin ; Z Xiao ; J Andrae ; Karl Tryggvason ; Tore Samuelsson ; Christer Betsholtz ; Minoru Takemoto
Kidney International (1523-1755). Vol. 71 (2007), 9, p. 889-900.
[Artikel, refereegranskad vetenskaplig]

The kidney glomerulus plays a crucial role in blood filtration but the molecular composition and physiology of the glomerulus is not well understood. We previously constructed and large-scale sequenced four mouse glomerular expressed sequence tag (EST) libraries from newborn and adult mouse glomeruli. Here, we compared glomerular EST profiles with whole kidney EST profiles, thereby identifying 497 transcripts corresponding to UniGene clusters that were glomerulus-enriched, that is expressed more abundantly in glomeruli than in whole kidney. These include several known protein-coding glomerulus-specific transcripts critical for glomerulus development and function, but also a large number of gene transcripts, which have not previously been shown to be expressed in the glomerulus, or implicated in glomerular functions. We used in situ hybridization to demonstrate glomerulus-specific RNA expression for six novel glomerular genes and the public Human Protein Atlas to verify glomerular protein expression for another two. The higher mRNA abundance for the eight genes in glomeruli compared with whole kidney was also verified by Taqman quantitative polymerase chain reaction. We surmise that the further characterization of these genes and proteins will increase our understanding of glomerular development and physiology.

Nyckelord: glomerulus, transcriptional profiling, podocyte, genetics and development, gene expression, cell biology and structure



Denna post skapades 2007-10-02. Senast ändrad 2015-08-25.
CPL Pubid: 50830

 

Institutioner (Chalmers)

Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi (GU)

Ämnesområden

Cell- och molekylärbiologi

Chalmers infrastruktur