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In vivo biocompatibility of bacterial cellulose

Gisela Helenius ; Henrik Bäckdahl ; Aase Bodin (Institutionen för kemi- och bioteknik) ; Ulf Nannmark ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Bo Risberg
Journal of biomedical materials research (1549-3296). Vol. 76 (2006), 2, p. 431-8.
[Artikel, refereegranskad vetenskaplig]

The biocompatibility of a scaffold for tissue engineered constructs is essential for the outcome. Bacterial cellulose (BC) consists of completely pure cellulose nanofibrils synthesized by Acetobacter xylinum. BC has high mechanical strength and can be shaped into three-dimensional structures. Cellulose-based materials induce negligible foreign body and inflammatory responses and are considered as biocompatible. The in vivo biocompatibility of BC has never been evaluated systematically. Thus, in the development of tissue engineered constructs with a BC scaffold, it is necessary to evaluate the in vivo biocompatibility. BC was implanted subcutaneously in rats for 1, 4, and 12 weeks. The implants were evaluated in aspects of chronic inflammation, foreign body responses, cell ingrowth, and angiogenesis, using histology, immunohistochemistry, and electron microscopy. There were no macroscopic signs of inflammation around the implants. There were no microscopic signs of inflammation either (i.e., a high number of small cells around the implants or the blood vessels). No fibrotic capsule or giant cells were present. Fibroblasts infiltrated BC, which was well integrated into the host tissue, and did not elicit any chronic inflammatory reactions. The biocompatibility of BC is good and the material has potential to be used as a scaffold in tissue engineering.

Nyckelord: Animals, Cell Proliferation/drug effects, Cellulose/adverse effects/*pharmacology/therapeutic use, Fibroblasts/cytology, Foreign-Body Reaction/chemically induced, Gluconacetobacter xylinus/chemistry, Implants, Experimental/adverse effects/*standards, Inflammation/chemically induced, Materials Testing, Neovascularization, Physiologic/drug effects, Rats, Tissue Engineering



Denna post skapades 2007-09-20. Senast ändrad 2012-01-26.
CPL Pubid: 49001

 

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Institutioner (Chalmers)

Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning (GU)
Institutionen för kemi- och bioteknik (2005-2014)
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi (GU)
Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)

Ämnesområden

MEDICIN OCH HÄLSOVETENSKAP

Chalmers infrastruktur

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