CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Mechanical properties of bacterial cellulose and interactions with smooth muscle cells

Henrik Bäckdahl (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Gisela Helenius ; Aase Bodin (Institutionen för kemi- och bioteknik, Polymerteknologi) ; Ulf Nannmark ; Bengt R Johansson ; Bo Risberg ; Paul Gatenholm (Institutionen för kemi- och bioteknik, Polymerteknologi)
Biomaterials (0142-9612). Vol. 27 (2006), 9, p. 2141-2149.
[Artikel, refereegranskad vetenskaplig]

Tissue engineered blood vessels (TEBV) represent an attractive approach for overcoming reconstructive problems associated with vascular diseases by providing small calibre vascular grafts. The aim of this study has been to evaluate a novel biomaterial, bacterial cellulose (BC), as a potential scaffold for TEBV. The morphology of the BC pellicle grown in static culture was investigated with SEM. Mechanical properties of BC were measured in Krebs solution and compared with the properties of porcine carotid arteries and ePTFE grafts. Attachment, proliferation and ingrowth of human smooth muscle cells (SMC) on the BC were analysed in vitro. The BC pellicle had an asymmetric structure composed of a fine network of nanofibrils similar to a collagen network. The shape of the stress-strain response of BC is reminiscent of the stress-strain response of the carotid artery, most probably due to the similarity in architecture of the nanofibrill networks. SMC adhered to and proliferated on the BC pellicle; an ingrowth of up to 40 microm was seen after 2 weeks of culture. BC exhibit attractive properties for use in future TEBV.

Nyckelord: Adhesins, Bacterial/*pharmacology/*ultrastructure, Animals, *Blood Vessel Prosthesis, Carotid Arteries/cytology, Cell Adhesion, Cell Proliferation, Cells, Cultured, Cellulose/*pharmacology/*ultrastructure, Collagen/pharmacology/ultrastructure, Gluconacetobacter xylinus/metabolism, Myocytes, Smooth Muscle/cytology/*drug effects, Swine, Tensile Strength, Tissue Engineering

Denna post skapades 2007-09-20. Senast ändrad 2012-08-20.
CPL Pubid: 48942


Läs direkt!

Länk till annan sajt (kan kräva inloggning)

Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Polymerteknologi (2005-2014)
Institutionen för kliniska vetenskaper, sektionen för kirurgi och kirurgisk gastroforskning (GU)
Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi (GU)



Chalmers infrastruktur

Relaterade publikationer

Denna publikation ingår i:

Engineering the shape of bacterial cellulose and its use as blood vessel replacement

Biocompatibility evaluation of bioengineered bacterial cellulose scaffold for use in blood vessels tissue engineering