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NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase (TDH1)

Hadi Valadi (Institutionen för kemi och biovetenskap) ; Åsa Valadi ; R. Ansell ; Lena Gustafsson (Institutionen för kemi och biovetenskap, Molekylär bioteknik) ; Lennart Adler ; Joakim Norbeck (Institutionen för kemi och biovetenskap, Molekylär bioteknik) ; Anders Blomberg
Current Genetics (0172-8083). Vol. 45 (2004), 2, p. 90-95.
[Artikel, refereegranskad vetenskaplig]

A strain of Saccharomyces cerevisiae lacking the GPD2 gene, encoding one of the glycerol-3-phosphate dehydrogenases, grows slowly under anaerobic conditions, due to reductive stress caused by the accumulation of cytoplasmic NADH. We used 2D-PAGE to study the effect on global protein expression of reductive stress in the anaerobically grown gpd2Delta strain. The most striking response was a strongly elevated expression of Tdh1p, the minor isoform of glyceraldehyde-3-phosphate dehydrogenase. This increased expression could be reversed by the addition of acetoin, a NADH-specific redox sink, which furthermore largely restored anaerobic growth of the gpd2Delta strain. Additional deletion of the TDH1 gene (but not of TDH2 or TDH3) improved anaerobic growth of the gpd2Delta strain. We therefore propose that TDH1 has properties not displayed by the other TDH isogenes and that its expression is regulated by reductive stress caused by an excess of cytoplasmic NADH.

Nyckelord: yeast, anaerobic growth, redox, reductive stress, gpd2, 2d-page, gts1 gene-product, glycerol formation, redox balances, yeast-cells, metabolism, protein, adaptation, mutants



Denna post skapades 2007-04-19. Senast ändrad 2011-01-20.
CPL Pubid: 40676

 

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Institutioner (Chalmers)

Institutionen för kemi och biovetenskap (1900-2005)
Institutionen för cell- och molekylärbiologi (1994-2011)
Institutionen för kemi och biovetenskap, Molekylär bioteknik (2002-2004)

Ämnesområden

Cell- och molekylärbiologi

Chalmers infrastruktur