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High resolution X-ray structures of the oxidised and reduced forms of nitrite reductase from Rhodobacter sphaeroides 2.4.3

Frida Jacobson (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; K Guo ; Kenneth Olesen ; Mats Ökvist ; Richard Neutze (Institutionen för kemi- och bioteknik, Molekylär bioteknik) ; Lennart Sjölin
Acta Crystallography D (0907-4449). Vol. 61 (2005), p. 1190-1198.
[Artikel, refereegranskad vetenskaplig]

Nitrite reductase is an enzyme operating in the denitrification pathway which catalyses the conversion of nitrite (NO2(-)) to gaseous nitric oxide (NO). Here, crystal structures of the oxidized and reduced forms of the copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3 are presented at 1.74 and 1.85 A resolution, respectively. Whereas the structure of the enzyme is very similar to those of other copper-containing nitrite reductases, folding as a trimer and containing two copper sites per monomer, the structures reported here enable conformational differences between the oxidized and reduced forms of the enzyme to be identified. In the type 1 copper site, a rotational perturbation of the side chain of the copper ligand Met182 occurs upon reduction. At the type 2 copper site, a dual conformation of the catalytic residue His287 is observed in the oxidized structure but is lacking in the reduced structure, such that the interactions of the oxidized type 2 copper ion can be regarded as adopting octahedral geometry. These findings shed light on the structural mechanism of the reduction of a copper-bound nitrite to nitric oxide and water.

Nyckelord: Copper, chemistry, Crystallography, X-Ray, Hydrogen-Ion Concentration, Nitrite Reductases, chemistry, metabolism, Oxidation-Reduction, Protein Conformation, Rhodobacter sphaeroides, enzymology

Denna post skapades 2007-01-17. Senast ändrad 2012-08-20.
CPL Pubid: 25762


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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Molekylär bioteknik (2005-2007)
Institutionen för medicinsk och fysiologisk kemi (1991-2005)
Institutionen för kemi (2001-2011)



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