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Applying microfluidic systems to study effects of glucose at single-cell level

Niek Welkenhuysen ; Caroline B. Adiels ; Mattias Goksör ; Stefan Hohmann (Institutionen för biologi och bioteknik)
Methods in Molecular Biology (1064-3745). Vol. 1713 (2018), p. 109-121.
[Artikel, refereegranskad vetenskaplig]

Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.

Nyckelord: Fluorescence microscopy , Glucose uptake , Microbiology , Microfluidic system , Optical tweezers , Saccharomyces cerevisiae , Single cell analysis



Denna post skapades 2018-01-04.
CPL Pubid: 254286

 

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Institutioner (Chalmers)

Institutionen för biologi och bioteknik

Ämnesområden

Biokemi och molekylärbiologi

Chalmers infrastruktur