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High gravity lignocellulose bioprocess development for ethanol and lactic acid production by multi-feed simultaneous saccharification and fermentation

Carl Johan Franzén (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Ruifei Wang (Institutionen för biologi och bioteknik, Industriell bioteknik) ; David Benjamin Nickel (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Lisbeth Olsson (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Salvatore Fusco (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Martina Aulitto (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Simonetta Bartolucci ; Patrizia Contursi
Oral presentation at: Recent Advances in Fermentation Technology, RAFT12. Oct 29 - Nov 1, Bonita Springs, FL, USA. (2017)
[Konferensbidrag, övrigt]

Second generation bioethanol production is becoming established in production plants across the world. The process can also be viewed as a model biorefinery concept for biotechnological conversion of recalcitrant lignocellulosic raw materials to chemicals and other products. We have developed a Multi-Feed SSCF process: a systematic, model-driven design of fed-batch simultaneous saccharification and co-fermentation of steam-pretreated lignocellulosic materials in standard stirred tank reactors. The design includes feeding of solid substrate, enzymes, and active, robust cell factories adapted to the present substrate. The concept has been applied not only to ethanol production with S. cerevisiae, but also to lactic acid production from wheat straw by the thermophilic, cellulolytic strain Bacillus coagulans MA-13, isolated from bean processing waste. High Gravity operation, i.e. fermentation at high concentrations of water insoluble solids (WIS), pushes the process towards higher product concentrations and productivities, and improved energy and water economy. By using the multi-feed SSCF approach, the ethanol process was pushed towards final product concentrations above 60 g/L, at about 90% of the theoretical yields on consumed substrate, using 22% w/w accumulated WIS additions of acid- and steam explosion-pretreated wheat straw. Bacillus coagulans MA-13 was found to secrete cellulolytic enzymes and ferment lignocellulose-derived sugars to lactic acid; thus, it may be a potential platform for consolidated bioprocessing of lactic acid. We investigated its performance in multi-feed SSF and found that pre-adaptation of cells to the liquid fraction of the steam-pretreated lignocellulosic material improves lactate productivity and reduces the SSF time from 33 to 12 hours.

Nyckelord: Lignocellulose, bioethanol, lactic acid, kinetic modeling, fed-batch, high gravity, scale-up

Contributing authors: Ruifei Wang, David Nickel, Lisbeth Olsson, Salvatore Fusco, Martina Aulitto, Simonetta Bartolucci, Patrizia Contursi

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Denna post skapades 2017-12-18. Senast ändrad 2017-12-18.
CPL Pubid: 253850