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Quantification of the Intracellular Life Time of Water Molecules to Measure Transport Rates of Human Aquaglyceroporins.

Madelene Palmgren ; Malin Hernebring ; Stefanie Eriksson ; Karin Elbing ; Cecilia Geijer (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Samo Lasič ; Peter Dahl ; Jesper S Hansen ; Daniel Topgaard ; Karin Lindkvist-Petersson
The Journal of membrane biology (1432-1424). Vol. 250 (2017), 6, p. 629-639.
[Artikel, refereegranskad vetenskaplig]

Orthodox aquaporins are transmembrane channel proteins that facilitate rapid diffusion of water, while aquaglyceroporins facilitate the diffusion of small uncharged molecules such as glycerol and arsenic trioxide. Aquaglyceroporins play important roles in human physiology, in particular for glycerol metabolism and arsenic detoxification. We have developed a unique system applying the strain of the yeast Pichia pastoris, where the endogenous aquaporins/aquaglyceroporins have been removed and human aquaglyceroporins AQP3, AQP7, and AQP9 are recombinantly expressed enabling comparative permeability measurements between the expressed proteins. Using a newly established Nuclear Magnetic Resonance approach based on measurement of the intracellular life time of water, we propose that human aquaglyceroporins are poor facilitators of water and that the water transport efficiency is similar to that of passive diffusion across native cell membranes. This is distinctly different from glycerol and arsenic trioxide, where high glycerol transport efficiency was recorded.



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Denna post skapades 2017-12-18.
CPL Pubid: 253846

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för biologi och bioteknik, Industriell bioteknik

Ämnesområden

Livsvetenskaper
Biokemi och molekylärbiologi

Chalmers infrastruktur