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Second harmonic generation for collagen I characterization in rectal cancer patients with and without preoperative radiotherapy

Stephanie Blockhuys (Institutionen för biologi och bioteknik, Kemisk biologi) ; Nisha Rani Agarwal (Institutionen för biologi och bioteknik, Kemisk biologi) ; C. Hildesjo ; I. Jarlsfelt ; Pernilla Wittung-Stafshede (Institutionen för biologi och bioteknik, Kemisk biologi) ; X. F. Sun
Journal of Biomedical Optics (1083-3668). Vol. 22 (2017), 10,
[Artikel, refereegranskad vetenskaplig]

Rectal cancer is treated with preoperative radiotherapy (RT) to downstage the tumor, reduce local recurrence, and improve patient survival. Still, the treatment outcome varies significantly and new biomarkers are desired. Collagen I (Col-I) is a potential biomarker, which can be visualized label-free by second harmonic generation (SHG). Here, we used SHG to identify Col-I changes induced by RT in surgical tissue, with the aim to evaluate the clinical significance of RT-induced Col-I changes. First, we established a procedure for quantitative evaluation of Col-I by SHG in CDX2-stained tissue sections. Next, we evaluated Col-I properties in material from 31 non-RT and 29 RT rectal cancer patients. We discovered that the Col-I intensity and anisotropy were higher in the tumor invasive margin than in the inner tumor and normal mucosa, and RT increased and decreased the intensity in inner tumor and normal mucosa, respectively. Furthermore, higher Col-I intensity in the inner tumor was related to increased distant recurrence in the non-RT group but to longer survival in the RT group. In conclusion, we present a new application of SHG for quantitative analysis of Col-I in surgical material, and the first data suggest Col-I intensity as a putative prognostic biomarker in rectal cancer.

Nyckelord: collagen I, second harmonic generation, prognosis, rectal cancer, radiotherapy

Denna post skapades 2017-11-22. Senast ändrad 2017-11-22.
CPL Pubid: 253302


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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Kemisk biologi


Biokemi och molekylärbiologi

Chalmers infrastruktur