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Exploiting off-targeting in guide-RNAs for CRISPR systems for simultaneous editing of multiple genes

Raphael Ferreira (Institutionen för biologi och bioteknik, Systembiologi) ; Francesco Gatto (Institutionen för biologi och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi)
FEBS Letters (0014-5793). Vol. 591 (2017), 20, p. 3288-3295.
[Artikel, refereegranskad vetenskaplig]

Bioinformatics tools to design guide-RNAs (gRNAs) in Clustered Regularly Interspaced Short Palindromic Repeats systems mostly focused on minimizing off-targeting to enhance efficacy of genome editing. However, there are circumstances in which off-targeting might be desirable to target multiple genes simultaneously with a single gRNA. We termed these gRNAs as promiscuous gRNAs. Here, we present a computational workflow to identify promiscuous gRNAs that putatively bind to the region of interest for a defined list of genes in a genome. We experimentally validated two promiscuous gRNA for gene deletion, one targeting FAA1 and FAA4 and one targeting PLB1 and PLB2, thus demonstrating that multiplexed genome editing through design of promiscuous gRNA can be performed in a time and cost-effective manner.

Nyckelord: computational tool, CRISPR, synthetic biology

Denna post skapades 2017-11-17.
CPL Pubid: 253217


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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi


Biokemi och molekylärbiologi

Chalmers infrastruktur