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Metabolic engineering of yeast for fermentative production of flavonoids

A. Rodriguez ; T. Strucko ; S. G. Stahlhut ; M. Kristensen ; D. K. Svenssen ; J. Forster ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi) ; I. Borodina
Bioresource Technology (0960-8524). Vol. 245 (2017), p. 1645-1654.
[Artikel, refereegranskad vetenskaplig]

Yeast Saccharomyces cerevisiae was engineered for de novo production of six different flavonoids (naringenin, liquiritigenin, kaempferol, resokaempferol, quercetin, and fisetin) directly from glucose, without supplementation of expensive intermediates. This required reconstruction of long biosynthetic pathways, comprising up to eight heterologous genes from plants. The obtained titers of kaempferol 26.57 +/- 2.66 mg L-1 and quercetin 20.38 +/- 2.57 mg L-1 exceed the previously reported titers in yeast. This is also the first report of de novo biosynthesis of resokaempferol and fisetin in yeast. The work demonstrates the potential of flavonoid-producing yeast cell factories.

Nyckelord: Flavonoids, Kaempferol, Resokaempferol, Fisetin, Saccharomyces cerevisiae



Denna post skapades 2017-11-08. Senast ändrad 2017-11-08.
CPL Pubid: 253008

 

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Institutioner (Chalmers)

Institutionen för biologi och bioteknik, Systembiologi

Ämnesområden

Mikrobiologi

Chalmers infrastruktur