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Fluorescence microscopy of nanochannel-confined DNA

Fredrik Westerlund (Institutionen för biologi och bioteknik, Kemisk biologi) ; F. Persson ; Joachim Fritzsche (Institutionen för fysik, Kemisk fysik (Chalmers)) ; J.P. Beech ; J.O. Tegenfeldt
Single Molecule Analysis p. 173-198. (2018)
[Kapitel]

Stretching of DNA in nanoscale confinement allows for several important studies. The genetic contents of the DNA can be visualized on the single DNA molecule level and both the polymer physics of confined DNA and also DNA/protein and other DNA/DNA-binding molecule interactions can be explored. This chapter describes the basic steps to fabricate the nanostructures, perform the experiments and analyze the data.

Nyckelord: DNA , Fluorescence , Nanochannels , Single molecule



Denna post skapades 2017-10-25.
CPL Pubid: 252744

 

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