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Sequential extraction of gel-forming proteins, collagen and collagen hydrolysate from gutted silver carp (Hypophthalmichthys molitrix), a biorefinery approach

Mehdi Abdollahi (Institutionen för biologi och bioteknik, Livsmedelsvetenskap) ; Masoud Rezaei ; Jafarpour Ali ; Ingrid Undeland (Institutionen för biologi och bioteknik, Livsmedelsvetenskap)
Food Chemistry (0308-8146). Vol. 242 (2018), p. 568-578.
[Artikel, refereegranskad vetenskaplig]

Collagen and collagen hydrolysate (CH) was recovered from the bone and skin containing sediment residue emerging during pH-shift-based protein isolation from silver carp. Hydrolysis resulted in higher yield (15.1–15.4%) compared to collagen isolation by acid or pepsin (3.1–5.9%) (p < 0.05). Isolated collagens were characterized as type I and maintained their triple-helical structure, confirmed by SDS-PAGE and FTIR. Pepsin-hydrolysis and sequential hydrolysis by pepsin and trypsin hydrolyzed all heavy molecular weight chains of collagen but sequential hydrolysis yielded higher degree of hydrolysis. When CH was added to a silver carp protein isolate prior to gelation, the gel behavior was dependent on molecular weight of the added CH. More hydrolyzed collagen emerging from sequential hydrolysis improved water holding capacity of the gel while reducing its breaking force. Thus, residue from pH-shift processing of fish can be used for isolation of high quality collagen/CH and provides a promising basis for a multiple-product fish biorefinery.

Nyckelord: Collagen, Hydrolysate, pH-shift method, Protein isolate, Silver carp, Biorefinery

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Denna post skapades 2017-09-27. Senast ändrad 2017-11-14.
CPL Pubid: 252084


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Institutionen för biologi och bioteknik, Livsmedelsvetenskap



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