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Computational discovery and functional validation of novel fluoroquinolone resistance genes in public metagenomic data sets

Fredrik Boulund (Institutionen för matematiska vetenskaper, Tillämpad matematik och statistik) ; Fanny Berglund (Institutionen för matematiska vetenskaper) ; C. F. Flach ; J. Bengtsson-Palme ; N. P. Marathe ; D. G. J. Larsson ; Erik Kristiansson (Institutionen för matematiska vetenskaper, Tillämpad matematik och statistik)
Bmc Genomics (1471-2164). Vol. 18 (2017), p. Art 682.
[Artikel, refereegranskad vetenskaplig]

Background: Fluoroquinolones are broad-spectrum antibiotics used to prevent and treat a wide range of bacterial infections. Plasmid-mediated qnr genes provide resistance to fluoroquinolones in many bacterial species and are increasingly encountered in clinical settings. Over the last decade, several families of qnr genes have been discovered and characterized, but their true prevalence and diversity still remain unclear. In particular, environmental and host-associated bacterial communities have been hypothesized to maintain a large and unknown collection of qnr genes that could be mobilized into pathogens. Results: In this study we used computational methods to screen genomes and metagenomes for novel qnr genes. In contrast to previous studies, we analyzed an almost 20-fold larger dataset comprising almost 13 terabases of sequence data. In total, 362,843 potential qnr gene fragments were identified, from which 611 putative qnr genes were reconstructed. These gene sequences included all previously described plasmid-mediated qnr gene families. Fifty-two of the 611 identified qnr genes were reconstructed from metagenomes, and 20 of these were previously undescribed. All of the novel qnr genes were assembled from metagenomes associated with aquatic environments. Nine of the novel genes were selected for validation, and six of the tested genes conferred consistently decreased susceptibility to ciprofloxacin when expressed in Escherichia coli. Conclusions: The results presented in this study provide additional evidence for the ubiquitous presence of qnr genes in environmental microbial communities, expand the number of known qnr gene variants and further elucidate the diversity of this class of resistance genes. This study also strengthens the hypothesis that environmental bacterial communities act as sources of previously uncharacterized qnr genes.

Nyckelord: Qnr, Fluroquinolone resistance, Horizontal gene transfer, Hidden Markov models, Metagenomics, Mediated Quinolone Resistance, Antibiotic-Resistance, Human Microbiome

Denna post skapades 2017-09-25. Senast ändrad 2017-10-10.
CPL Pubid: 252050


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Institutionen för matematiska vetenskaper, Tillämpad matematik och statistikInstitutionen för matematiska vetenskaper, Tillämpad matematik och statistik (GU)
Institutionen för matematiska vetenskaperInstitutionen för matematiska vetenskaper (GU)



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