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Bactericidal effect of photocatalytically-active nanostructured TiO2 surfaces on biofilms of the early oral colonizer, Streptococcus oralis

Emma Westas (Institutionen för kemi och kemiteknik, Teknisk ytkemi) ; M. Hayashi ; F. Cecchinato ; A. Wennerberg ; Martin Andersson (Institutionen för kemi och kemiteknik, Teknisk ytkemi) ; R. Jimbo ; J. R. Davies
Journal of Biomedical Materials Research Part A (1549-3296). Vol. 105 (2017), 8, p. 2321-2328.
[Artikel, refereegranskad vetenskaplig]

This study evaluated the photocatalytic bactericidal effect of nanostructured anatase-rich titanium dioxide (TiO2) on microbial biofilms. Commercially pure titanium discs were spin-coated with photocatalytic TiO2 nanoparticles (P25). Uncoated discs were used as control (CTRL). Half of the CTRL and half of the P25-coated surfaces were coated with purified saliva (SAL) to give four different groups (CTRL, CTRL + SAL, P25 and P25 + SAL). Streptococcus oralis were allowed to form biofilms on the discs for 18 h and non-adherent cells were rinsed off. Bacterial viability was assessed at time 0 with Live/Dead BacLight staining and epifluorescence microscopy. The remaining discs were divided into a non-UV group and UVA-irradiated (1UV) group (irradiation time, 6 or 24 h). Thereafter, viability was assessed as above. Viability at time 0 was high and no dead cells were seen on any of the surfaces, even after 24 h, in the absence of UVA. However, after 24 h of exposure, the proportion of viable cells was reduced by 40% on the P25 discs compared to 0 and 6 h, and this effect was enhanced with a salivary pellicle. Members of mixed species biofilms differ in their susceptibility to the bactericidal effect of the surfaces tested and further investigations are needed to optimize the conditions.

Nyckelord: photocatalyst, ultraviolet A, bactericidal effect, titanium implant, biofilm



Denna post skapades 2017-08-14.
CPL Pubid: 251079

 

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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Teknisk ytkemi

Ämnesområden

Medicinska material och protesteknik

Chalmers infrastruktur