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Exploring the potential of Saccharomyces cerevisiae for biopharmaceutical protein production

Guokun Wang (Institutionen för biologi och bioteknik, Systembiologi) ; Mingtao Huang (Institutionen för biologi och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi)
Current Opinion in Biotechnology (09581669). Vol. 48 (2017), p. 77-84.
[Artikel, refereegranskad vetenskaplig]

Production of recombinant proteins by yeast plays a vital role in the biopharmaceutical industry. It is therefore desirable to develop yeast platform strains for over-production of various biopharmaceutical proteins, but this requires fundamental knowledge of the cellular machinery, especially the protein secretory pathway. Integrated analyses of multi-omics datasets can provide comprehensive understanding of cellular function, and can enable systems biology-driven and mathematical model-guided strain engineering. Rational engineering and introduction of trackable genetic modifications using synthetic biology tools, coupled with high-throughput screening are, however, also efficient approaches to relieve bottlenecks hindering high-level protein production. Here we review advances in systems biology and metabolic engineering of yeast for improving recombinant protein production.

Nyckelord: Biology; Functions; Machinery; Metabolic engineering; Recombinant proteins; Throughput; Yeast

Denna post skapades 2017-06-14.
CPL Pubid: 249830


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