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Autophagy induction regulates influenza virus replication in a time-dependent manner

Neda Feizi ; Parvaneh Mehrbod ; Bizhan Romani ; Hoorieh Soleimanjahi ; Taravat Bamdad ; Amir Feizi (Institutionen för biologi och bioteknik, Systembiologi) ; Ehsan Ollah Jazaeri ; Hadiseh Shokouhi Targhi ; Maryam Saleh ; Abbas Jamali ; Fatemeh Fotouhi ; Reza Nasrollahi Nargesabad ; Asghar Abdoli
Journal of Medical Microbiology (00222615). Vol. 66 (2017), 4, p. 536-541.
[Artikel, refereegranskad vetenskaplig]

Purpose. Autophagy plays a key role in host defence responses against microbial infections by promoting degradation of pathogens and participating in acquired immunity. The interaction between autophagy and viruses is complex, and this pathway is hijacked by several viruses. Influenza virus (IV) interferes with autophagy through its replication and increases the accumulation of autophagosomes by blocking lysosome fusion. Thus, autophagy could be an effective area for antiviral research. Methodology. In this study, we evaluated the effect of autophagy on IV replication. Two cell lines were transfected with Beclin-1 expression plasmid before (prophylactic approach) and after (therapeutic approach) IV inoculation. Results/Key findings. Beclin-1 overexpression in the cells infected by virus induced autophagy to 26 %. The log10 haemagglutinin titre and TCID50 (tissue culture infective dose giving 50% infection) of replicating virus were measured at 24 and 48 h post-infection. In the prophylactic approach, the virus titre was enhanced significantly at 24 h post-infection (P?0.01), but it was not significantly different from the control at 48 h post-infection. In contrast, the therapeutic approach of autophagy induction inhibited the virus replication at 24 and 48 h post-infection. Additionally, we showed that inhibition of autophagy using 3-methyladenine reduced viral replication. Conclusion. This study revealed that the virus (H1N1) titre was controlled in a time-dependent manner following autophagy induction in host cells. Manipulation of autophagy during the IV life cycle can be targeted both for antiviral aims and for increasing viral yield for virus production.

Nyckelord: Autophagy; Beclin-1; Influenza virus; Replication

Denna post skapades 2017-06-14. Senast ändrad 2017-07-12.
CPL Pubid: 249807


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