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Immobilisation on mesoporous silica and solvent rinsing improve the transesterification abilities of feruloyl esterases from Myceliophthora thermophila

Silvia Hüttner (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Milene Zezzi Do Valle Gomes (Institutionen för biologi och bioteknik, Industriell bioteknik) ; Laura Iancu ; Anders Palmqvist (Institutionen för kemi och kemiteknik, Teknisk ytkemi) ; Lisbeth Olsson (Institutionen för biologi och bioteknik, Industriell bioteknik)
Bioresource Technology (0960-8524). Vol. 239 (2017), p. 57-65.
[Artikel, refereegranskad vetenskaplig]

The immobilisation of four feruloyl esterases (FAEs) (FaeA1, FaeA2, FaeB1, FaeB2) from the thermophilic fungus Myceliophthora thermophila C1 was studied and optimised via physical adsorption onto various mesoporous silica particles with pore diameters varying from 6.6 nm to 10.9 nm. Using crude enzyme preparations, enrichment of immobilised FAEs was observed, depending on pore diameter and protein size. The immobilised enzymes were successfully used for the synthesis of butyl ferulate through transesterification of methyl ferulate with 1-butanol. Although the highest butyl ferulate yields were obtained with free enzyme, the synthesis-to-hydrolysis ratio was higher when using immobilised enzymes. Over 90% of the initial activity was observed in a reusability experiment after nine reaction cycles, each lasting 24 h. Rinsing with solvent to remove water from the immobilised enzymes further improved their activity. This study demonstrates the suitability of immobilised crude enzyme preparations in the development of biocatalysts for esterification reactions.

Nyckelord: Biocatalysis; Butyl ferulate; Enzyme immobilisation; Esterification; SBA-15



Denna post skapades 2017-06-09. Senast ändrad 2017-10-03.
CPL Pubid: 249675

 

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