CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Nano Secondary Ion Mass Spectrometry Imaging of Dopamine Distribution Across Nanometer Vesicles

Jelena Lovrić (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Johan Dunevall (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Anna Larsson ; Lin Ren (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Shalini Andersson ; Anders Meibom ; Per Malmberg (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Michael E. Kurczy ; Andrew G Ewing (Institutionen för kemi och kemiteknik, Analytisk kemi)
ACS Nano (1936-0851). Vol. 11 (2017), 4, p. 3446-3455.
[Artikel, refereegranskad vetenskaplig]

We report an approach to spatially resolve the content across nanometer neuroendocrine vesicles in nerve-like cells by correlating super high-resolution mass spectrometry imaging, NanoSIMS, with transmission electron microscopy (TEM). Furthermore, intracellular electrochemical cytometry at nanotip electrodes is used to count the number of molecules in individual vesicles to compare to imaged amounts in vesicles. Correlation between the NanoSIMS and TEM provides nanometer resolution of the inner structure of these organelles. Moreover, correlation with electrochemical methods provides a means to quantify and relate vesicle neurotransmitter content and release, which is used to explain the slow transfer of dopamine between vesicular compartments. These nanoanalytical tools reveal that dopamine loading/unloading between vesicular compartments, dense core and halo solution, is a kinetically limited process. The combination of NanoSIMS and TEM has been used to show the distribution profile of newly synthesized dopamine across individual vesicles. Our findings suggest that the vesicle inner morphology might regulate the neurotransmitter release event during open and closed exocytosis from dense core vesicles with hours of equilibrium needed to move significant amounts of catecholamine from the protein dense core despite its nanometer size.

Nyckelord: electrochemistry; nanocompartments; nanoimaging; NanoSIMS; vesicle content



Denna post skapades 2017-06-09. Senast ändrad 2017-06-13.
CPL Pubid: 249652

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Analytisk kemi

Ämnesområden

Kemi

Chalmers infrastruktur