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Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds

ida Henriksson (Institutionen för kemi och kemiteknik, Polymerteknologi) ; Paul Gatenholm (Institutionen för kemi och kemiteknik, Polymerteknologi) ; Daniel Hägg (Institutionen för kemi och kemiteknik, Polymerteknologi ; Institutionen för medicin, avdelningen för molekylär och klinisk medicin)
Biofabrication (1758-5082). Vol. 9 (2017), 1, p. Article Number: 015022 .
[Artikel, refereegranskad vetenskaplig]

Compared to standard 2D culture systems, new methods for 3D cell culture of adipocytes could provide more physiologically accurate data and a deeper understanding of metabolic diseases such as diabetes. By resuspending living cells in a bioink of nanocellulose and hyaluronic acid, we were able to print 3D scaffolds with uniform cell distribution. After one week in culture, cell viability was 95%, and after two weeks the cells displayed a more mature phenotype with larger lipid droplets than standard 2D cultured cells. Unlike cells in 2D culture, the 3D bioprinted cells did not detach upon lipid accumulation. After two weeks, the gene expression of the adipogenic marker genes PPAR. and FABP4 was increased 2.0- and 2.2-fold, respectively, for cells in 3D bioprinted constructs compared with 2D cultured cells. Our 3D bioprinted culture system produces better adipogenic differentiation of mesenchymal stem cells and a more mature cell phenotype than conventional

Nyckelord: 3D culture, 3D bioprinting, adipocyte, adipose tissue, nanocellulose bioink, Supplementary material for, adipose-tissue, stem-cells, bacterial cellulose, culture, differentiation, model, bioink, preadipocytes, constructs, gelatin, Engineering, Materials Science



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Denna post skapades 2017-05-05. Senast ändrad 2017-06-30.
CPL Pubid: 249137

 

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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Polymerteknologi
Institutionen för medicin, avdelningen för molekylär och klinisk medicin (GU)

Ämnesområden

Livsvetenskaper
Kemi

Chalmers infrastruktur