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Light-sensing via hydrogen peroxide and a peroxiredoxin

Kristofer Bodvard (Institutionen för fysik, Bionanofotonik (Chalmers)) ; K. Peeters ; F. Roger ; N. Romanov ; A. Igbaria ; Niek Welkenhuysen (Institutionen för biologi och bioteknik, Systembiologi) ; G. Palais ; W. Reiter ; M. B. Toledano ; Mikael Käll (Institutionen för fysik, Bionanofotonik (Chalmers)) ; M. Molin
Nature Communications (2041-1723). Vol. 8 (2017),
[Artikel, refereegranskad vetenskaplig]

Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.

Nyckelord: yeast transcription factor, saccharomyces-cerevisiae, nuclear-localization, circadian-rhythms, protein-kinase, life-span, glucose starvation, gene disruption, core component, factor msn2p



Denna post skapades 2017-04-19. Senast ändrad 2017-10-03.
CPL Pubid: 248892

 

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