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Detailed characterization of plasmids carrying resistance genes using optical DNA mapping

Vilhelm Müller (Institutionen för biologi och bioteknik, Kemisk biologi) ; Lena Nyberg (Institutionen för biologi och bioteknik, Kemisk biologi) ; Saair Quaderi (Institutionen för biologi och bioteknik) ; E. Kristiansson ; T. Ambjörnsson ; L. Sandegren ; Fredrik Westerlund (Institutionen för biologi och bioteknik, Kemisk biologi)
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 p. 1561-1562. (2016)
[Konferensbidrag, refereegranskat]

We present an assay, based on optical DNA mapping in nanochannels that is capable of characterizing the plasmid content of bacterial isolates resistant to antibiotics in a fast an detailed way. In a single experiment we determine the number of different plasmids in each sample, their size, as well as a barcode that can be used for plasmid identification and tracing. In addition we demonstrate that we can identify resistance genes on individual plasmids using CRISPR/Cas9. We foresee that the assay can be a useful tool all the way from fundamental plasmid biology to diagnostics and surveillance of resistant infections.



Denna post skapades 2017-03-27. Senast ändrad 2017-06-27.
CPL Pubid: 248695