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Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils

Fredrik Ahrentorp ; Jakob Blomgren ; Christian Jonasson ; Anna Sarwe ; Sobhan Sepehri (Institutionen för mikroteknologi och nanovetenskap, Kvantkomponentfysik) ; Emil Eriksson (Institutionen för mikroteknologi och nanovetenskap) ; Alexei Kalaboukhov (Institutionen för mikroteknologi och nanovetenskap, Kvantkomponentfysik) ; Aldo Jesorka (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Dag Winkler (Institutionen för mikroteknologi och nanovetenskap, Kvantkomponentfysik) ; Justin F. Schneiderman ; Mats Nilsson ; Jan Albert ; Teresa Zardán Gómez de la Torre ; Maria Strømme ; Christer Johansson
Journal of Magnetism and Magnetic Materials (0304-8853). Vol. 427 (2017), p. 14-18.
[Artikel, refereegranskad vetenskaplig]

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

Nyckelord: AC susceptometry; Biodetection; Differential induction coil; Magnetic multi-core particles; Magnetic nanoparticles; RCA coil



Denna post skapades 2017-03-03.
CPL Pubid: 248412

 

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