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D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants

J. Wienstroer ; Martin Engqvist (Institutionen för kemi- och bioteknik, Systembiologi) ; H.H. Kunz ; U.I. Flügge ; V.G. Maurino
FEBS Letters (0014-5793). Vol. 586 (2012), 1, p. 36-40.
[Artikel, refereegranskad vetenskaplig]

d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

Nyckelord: Arabidopsis thaliana , d-Lactate dehydrogenase , Plant transformation , Selection marker



Denna post skapades 2017-02-13.
CPL Pubid: 248107

 

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Institutioner (Chalmers)

Institutionen för kemi- och bioteknik, Systembiologi (2008-2014)

Ämnesområden

Molekylärbiologi
Botanik

Chalmers infrastruktur