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FRET enhancement close to gold nanoparticles positioned in DNA origami constructs

Nesrine Aissaoui (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Kasper Moth-Poulsen (Institutionen för kemi och kemiteknik, Polymerteknologi) ; Mikael Käll (Institutionen för fysik, Bionanofotonik (Chalmers)) ; Peter Johansson ; Marcus Wilhelmsson (Institutionen för kemi och kemiteknik, Fysikalisk kemi) ; Bo Albinsson (Institutionen för kemi och kemiteknik, Fysikalisk kemi)
Nanoscale (2040-3364). Vol. 9 (2017), 2, p. 673-683.
[Artikel, refereegranskad vetenskaplig]

Here we investigate the energy transfer rates of a Förster resonance energy transfer (FRET) pair positioned in close proximity to a 5 nm gold nanoparticle (AuNP) on a DNA origami construct. We study the distance dependence of the FRET rate by varying the location of the donor molecule, D, relative to the AuNP while maintaining a fixed location of the acceptor molecule, A. The presence of the AuNP induces an alteration in the spontaneous emission of the donor (including radiative and non-radiative rates) which is strongly dependent on the distance between the donor and AuNP surface. Simultaneously, the energy transfer rates are enhanced at shorter D-A (and D-AuNP) distances. Overall, in addition to the direct influence of the acceptor and AuNP on the donor decay there is also a significant increase in decay rate not explained by the sum of the two interactions. This leads to enhanced energy transfer between donor and acceptor in the presence of a 5 nm AuNP. We also demonstrate that the transfer rate in the three "particle" geometry (D + A + AuNP) depends approximately linearly on the transfer rate in the donor-AuNP system, suggesting the possibility to control FRET process with electric field induced by 5 nm AuNPs close to the donor fluorophore. It is concluded that DNA origami is a very versatile platform for studying interactions between molecules and plasmonic nanoparticles in general and FRET enhancement in particular.

Nyckelord: Electric fields, Energy transfer, Gold, Metal nanoparticles, Molecules, Nanoparticles



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Denna post skapades 2017-01-26. Senast ändrad 2017-10-03.
CPL Pubid: 247724

 

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