CPL - Chalmers Publication Library
| Utbildning | Forskning | Styrkeområden | Om Chalmers | In English In English Ej inloggad.

Single cell amperometry reveals curcuminoids modulate the release of neurotransmitters during exocytosis from PC12 cells

X. C. Li ; Amir Saeid Mohammadi (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Andrew G Ewing (Institutionen för kemi och kemiteknik, Analytisk kemi)
Journal of Electroanalytical Chemistry (0022-0728). Vol. 781 (2016), p. 30-35.
[Artikel, refereegranskad vetenskaplig]

We used single cell amperometry to examine whether curcumin and bisdemethoxycurcumin (BDMC), substances that are suggested to affect learning and memory, can modulate monoamine release from PC12 cells. Our results indicate both curcumin and BDMC need long-term treatment (72 h in this study) to influence exocytosis effectively. By analyzing the parameters calculated from single exocytosis events, it can be concluded that curcumin and BDMC affect exocytosis through different mechanisms. Curcumin accelerates the event dynamics with no significant change of the monoamine amount released from single exocytotic events, whereas BDMC attenuates the amount from single exocytotic event with no significant change of the event dynamics. This comparison of the effect of curcumin and BDMC on exocytosis at the single cell level brings insight into their different mechanisms, which might lead to different biological actions. The effect of curcumin and BDMC on the opening and closing of the exocytotic fusion pore were also investigated. These results might be helpful for understanding the improvement of learning and memory and the anti-depression properties of curcuminoids.

Nyckelord: Single cell amperometry, Exocytosis, Curcumin, Bisdemethoxycurcumin, PC12 cells, Model cells



Denna post skapades 2017-01-23.
CPL Pubid: 247608

 

Läs direkt!


Länk till annan sajt (kan kräva inloggning)


Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Analytisk kemi

Ämnesområden

Elektrokemi

Chalmers infrastruktur