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Nonspecific Colloidal-Type Interaction Explains Size-Dependent Specific Binding of Membrane-Targeted Nanoparticles

Anders Lundgren (Institutionen för fysik, Biologisk fysik (Chalmers)) ; Björn Agnarsson (Institutionen för fysik, Biologisk fysik (Chalmers)) ; R. Zirbs ; Vladimir P. Zhdanov (Institutionen för fysik, Biologisk fysik (Chalmers)) ; E. Reimhult ; Fredrik Höök (Institutionen för fysik, Biologisk fysik (Chalmers))
ACS Nano (1936-0851). Vol. 10 (2016), 11, p. 9974-9982.
[Artikel, refereegranskad vetenskaplig]

Emerging biomedical applications such as molecular imaging and drug delivery often require directed binding of nanoparticles to cell-membrane receptors. The specific apparent affinity of such ligand-functionalized particles is size-dependent, an observation so far solely attributed to multivalent receptor ligand interaction. We question the universality of this explanation by demonstrating that the binding kinetics also depends on weak, attractive colloidal-type interaction between nanoparticles and a lipid membrane. Applying label-free single-particle imaging, we correlate binding of nanoparticles targeted to a cell-mimetic lipid membrane with the distribution of nontargeted particles freely diffusing close to the membrane interface. This analysis shows that already a weak, k(B) T-scale attraction present between 50 nm gold nanoparticles and the membrane renders these particles an order of magnitude higher avidity compared to 20 nm particles. A stronger emphasis on nonspecific particle membrane interaction might thus be required to accurately predict nanoparticle targeting and other similar processes such as cellular uptake of exosomes and viruses.

Nyckelord: targeted nanoparticles; lipid-membrane interaction; DLVO interaction; single-particle imaging; light-scattering; quartz crystal microbalance

Denna post skapades 2017-01-13.
CPL Pubid: 246837


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Institutionen för fysik, Biologisk fysik (Chalmers)



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