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Proteotyping: Proteomic characterization, classification and identification of microorganisms--A prospectus.

Roger Karlsson ; Lucia Gonzales-Siles ; Fredrik Boulund ; Liselott Svensson-Stadler ; Susann Skovbjerg ; Anders Karlsson ; Max Davidsson ; Stefan Hulth ; Erik Kristiansson (Institutionen för matematiska vetenskaper, matematisk statistik) ; Edward R.B. Moore
Systematic and Applied Microbiology (0723-2020). Vol. 38 (2015), 4, p. 246-257.
[Artikel, refereegranskad vetenskaplig]

Modern microbial systematics requires a range of methodologies for the comprehensive characterization, classification and identification of microorganisms. While whole-genome sequences provide the ultimate reference for defining microbial phylogeny and taxonomy, selected biomarker-based strategies continue to provide the means for the bulk of microbial systematic studies. Proteomics, the study of the expression of genes, as well as the structure and function of the resulting proteins, offers indirect measures of genome sequence data. Recent developments in applications of proteomics for analyzing microorganisms have paralleled the growing microbial genome sequence database, as well as the evolution of mass spectrometry (MS) instrumentation and bioinformatics. MALDI-TOF MS, which generates proteomic mass patterns for ‘fingerprint’-based characterizations, has provided a marked breakthrough for microbial identification. However, MALDI-TOF MS is limited in the number of targets that can be detected for strain characterization. Advanced methods of tandem mass spectrometry, in which proteins and peptides generated from proteins, are characterized and identified, using LC–MS/MS, provide the ability to detect hundreds or thousands of expressed microbial strain markers for high-resolution characterizations and identifications. Model studies demonstrate the application of proteomics-based analyses for bacterial species- and strain-level detection and identification and for characterization of environmentally relevant, metabolically diverse bacteria. Proteomics-based approaches represent an emerging complement to traditional methods of characterizing microorganisms, enabling the elucidation of the expressed biomarkers of genome sequence information, which can be applied to ‘proteotyping’ applications of microorganisms at all taxonomic levels.



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Denna post skapades 2016-12-06. Senast ändrad 2017-07-03.
CPL Pubid: 245904

 

Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för biomedicin, avdelningen för infektionssjukdomar (GU)
Institutionen för matematiska vetenskaper, matematisk statistik (2005-2016)

Ämnesområden

Livsvetenskaper
Hållbar utveckling
Mikrobiologi inom det medicinska området
Medicinsk teknik

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