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Harnessing yeast peroxisomes for biosynthesis of fatty-acid-derived biofuels and chemicals with relieved side-pathway competition

Yongjin Zhou (Institutionen för biologi och bioteknik, Systembiologi) ; Nicolaas Buijs (Institutionen för biologi och bioteknik, Systembiologi) ; Zhiwei Zhu (Institutionen för biologi och bioteknik, Systembiologi) ; Diego Orol Gómez (Institutionen för biologi och bioteknik, Systembiologi) ; Akarin Boonsombuti (Institutionen för biologi och bioteknik, Systembiologi) ; Verena Siewers (Institutionen för biologi och bioteknik, Systembiologi) ; Jens B. Nielsen (Institutionen för biologi och bioteknik, Systembiologi)
Journal of the American Chemical Society (0002-7863). Vol. 138 (2016), 47, p. 15368–15377.
[Artikel, refereegranskad vetenskaplig]

Establishing efficient synthetic pathways for microbial production of biochemicals is often hampered by competing pathways and/or insufficient precursor supply. Compartmentalization in cellular organelles can isolate synthetic pathways from competing pathways, and provide a compact and suitable environment for biosynthesis. Peroxisomes are cellular organelles where fatty acids are degraded, a process that is inhibited under typical fermentation conditions making them an interesting workhouse for production of fatty-acid-derived molecules. Here, we show that targeting synthetic pathways to peroxisomes can increase the production of fatty-acid-derived fatty alcohols, alkanes and olefins up to 700%. In addition, we demonstrate that biosynthesis of these chemicals in the peroxisomes results in significantly decreased accumulation of byproducts formed by competing enzymes. We further demonstrate that production can be enhanced up to 3-fold by increasing the peroxisome population. The strategies described here could be used for production of other chemicals, especially acyl-CoA-derived molecules.

Nyckelord: Biofuels, Fatty acid, Metabolic engineering

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Denna post skapades 2016-12-01. Senast ändrad 2017-06-27.
CPL Pubid: 245764


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