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Hydrodynamic Propulsion of Liposomes Electrostatically Attracted to a Lipid Membrane Reveals Size-Dependent Conformational Changes

S. R. Tabaei ; J. J. J. Gillissen ; Stephan Block (Institutionen för fysik, Biologisk fysik (Chalmers)) ; Fredrik Höök (Institutionen för fysik, Biologisk fysik (Chalmers)) ; N. J. Cho
Acs Nano (1936-0851). Vol. 10 (2016), 9, p. 8812-8820.
[Artikel, refereegranskad vetenskaplig]

The efficiency of lipid nanoparticle uptake across cellular membranes is strongly dependent on the very first interaction step. Detailed understanding of this step is in part hampered by the large heterogeneity in the physicochemical properties of lipid nanoparticles, such as liposomes, making conventional ensemble-averaging methods too blunt to address details of this complex process. Here, we contribute a means to explore whether individual liposomes become deformed upon binding to fluid cell-membrane mimics. This was accomplished by using hydrodynamic forces to control the propulsion of nanoscale liposomes electrostatically attracted to a supported lipid bilayer. In this way, the size of individual liposomes could be determined by simultaneously measuring both their individual drift velocity and diffusivity, revealing that for a radius of similar to 45 nm, a close agreement with dynamic light scattering data was observed, while larger liposomes (radius similar to 75 nm) displayed a significant deformation unless composed of a gel-phase lipid. The relevance of being able to extract this type of information is discussed in the context of membrane fusion and cellular uptake.

Nyckelord: liposome deformation, single particle tracking, TIRF microscopy, supported lipid bilayer, hydrodynamic, single-particle tracking, synaptic vesicle fusion, bilayer formation, optical measurements, supported bilayer, shear-flow, curvature, cells, nanoparticles, deformation, Chemistry, Science & Technology - Other Topics, Materials Science, gier j, 1993, chemistry and physics of lipids, v64, p187

Denna post skapades 2016-10-28. Senast ändrad 2017-10-03.
CPL Pubid: 244426


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