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Cholesterol Alters the Dynamics of Release in Protein Independent Cell Models for Exocytosis

Neda Najafinobar (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Lisa J. Mellander ; Michael E. Kurczy (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Johan Dunevall (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Tina B. Angerer ; John S. Fletcher (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Ann-Sofie Cans (Institutionen för kemi och kemiteknik, Analytisk kemi)
Scientific Reports (2045-2322). Vol. 6 (2016), Article number: 33702,
[Artikel, refereegranskad vetenskaplig]

Neurons communicate via an essential process called exocytosis. Cholesterol, an abundant lipid in both secretory vesicles and cell plasma membrane can affect this process. In this study, amperometric recordings of vesicular dopamine release from two different artificial cell models created from a giant unilamellar liposome and a bleb cell plasma membrane, show that with higher membrane cholesterol the kinetics for vesicular release are decelerated in a concentration dependent manner. This reduction in exocytotic speed was consistent for two observed modes of exocytosis, full and partial release. Partial release events, which only occurred in the bleb cell model due to the higher tension in the system, exhibited amperometric spikes with three distinct shapes. In addition to the classic transient, some spikes displayed a current ramp or plateau following the maximum peak current. These post spike features represent neurotransmitter release from a dilated pore before constriction and show that enhancing membrane rigidity via cholesterol adds resistance to a dilated pore to re-close. This implies that the cholesterol dependent biophysical properties of the membrane directly affect the exocytosis kinetics and that membrane tension along with membrane rigidity can influence the fusion pore dynamics and stabilization which is central to regulation of neurochemical release.

Nyckelord: individual chromaffin cells, flickering fusion pores, kiss-and-run, membrane tension, bending elasticity, lipid nanotubes, snare proteins, pc12 cells, vesicles, hemifusion

Denna post skapades 2016-10-14. Senast ändrad 2017-01-27.
CPL Pubid: 243430


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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Analytisk kemi
Institutionen för kemi och molekylärbiologi (GU)



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