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Using Single-Cell Amperometry To Reveal How Cisplatin Treatment Modulates the Release of Catecholamine Transmitters during Exocytosis

Xianchan Li ; Johan Dunevall (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Andrew G Ewing (Institutionen för kemi och molekylärbiologi ; Institutionen för kemi och kemiteknik, Analytisk kemi)
Angewandte Chemie-International Edition (1433-7851). Vol. 55 (2016), 31, p. 9041-9044.
[Artikel, refereegranskad vetenskaplig]

The pretreatment of cultured pheochromocytoma (PC12) cells with cis-diamminedichloroplatinum (cisplatin), an anti-cancer drug, influences the exocytotic ability of the cells in a dose-dependent manner. Low concentrations of cisplatin stimulate catecholamine release whereas high concentrations inhibit it. Single-cell amperometry reflects that 2 mm cisplatin treatment increases the frequency of exocytotic events and reduces their duration, whereas 100 mm cisplatin treatment decreases the frequency of exocytotic events and increases their duration. Furthermore, the stability of the initial fusion pore that is formed in the lipid membrane during exocytosis is also regulated differentially by different cisplatin concentrations. This study thus suggests that cisplatin influences exocytosis by multiple mechanisms.

Nyckelord: amperometry, chemotherapy, cisplatin, exocytosis



Denna post skapades 2016-10-14.
CPL Pubid: 243398

 

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Institutioner (Chalmers)

Institutionen för kemi och molekylärbiologi (GU)
Institutionen för kemi och kemiteknik, Analytisk kemi

Ämnesområden

Biokemi och molekylärbiologi

Chalmers infrastruktur