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**Harvard**

Rupert, D., Shelke, G., Emilsson, G., Claudio, V., Block, S., Lässer, C., Dahlin, A., Lötvall, J., Bally, M., Zhdanov, V. och Höök, F. (2016) *Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles*.

** BibTeX **

@article{

Rupert2016,

author={Rupert, Déborah L. M. and Shelke, Ganesh V and Emilsson, Gustav and Claudio, Virginia and Block, Stephan and Lässer, Cecilia and Dahlin, Andreas and Lötvall, Jan and Bally, Marta and Zhdanov, Vladimir P. and Höök, Fredrik},

title={Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles},

journal={Analytical Chemistry},

issn={0003-2700},

volume={88},

issue={20},

pages={ 9980-9988},

abstract={Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role and of potentially using them as disease biomarkers. In principle, this can be achieved by measuring the rate of diffusion-limited mass uptake to a sensor surface modified with a receptor designed to only bind the subpopulation of interest. However, a significant error is introduced if the targeted EV subpopulation has a size, and thus hydrodynamic diffusion coefficient, that differs from the mean size and diffusion coefficient of the whole EV population and/or if the EVs become deformed upon binding to the surface. We here demonstrate a new approach to determine the mean size (or effective film thickness) of bound nanoparticles, in general, and EV subpopulation carrying a marker of interest, in particular. The method is based on operating surface plasmon resonance simultaneously at two wavelengths with different sensing depths and using the ratio of the corresponding responses to extract the particle size on the surface. By estimating in this way the degree of deformation of adsorbed EVs, we markedly improved their bulk concentration determination and showed that EVs carrying the exosomal marker CD63 correspond to not more than around 10% of the EV sample.},

year={2016},

keywords={Dual-Wavelength Surface Plasmon Resonance, Extracellular Vesicles},

}

** RefWorks **

RT Journal Article

SR Electronic

ID 243377

A1 Rupert, Déborah L. M.

A1 Shelke, Ganesh V

A1 Emilsson, Gustav

A1 Claudio, Virginia

A1 Block, Stephan

A1 Lässer, Cecilia

A1 Dahlin, Andreas

A1 Lötvall, Jan

A1 Bally, Marta

A1 Zhdanov, Vladimir P.

A1 Höök, Fredrik

T1 Dual-Wavelength Surface Plasmon Resonance for Determining the Size and Concentration of Sub-Populations of Extracellular Vesicles

YR 2016

JF Analytical Chemistry

SN 0003-2700

VO 88

IS 20

SP 9980

OP 9988

AB Accurate concentration determination of subpopulations of extracellular vesicles (EVs), such as exosomes, is of importance both in the context of understanding their fundamental biological role and of potentially using them as disease biomarkers. In principle, this can be achieved by measuring the rate of diffusion-limited mass uptake to a sensor surface modified with a receptor designed to only bind the subpopulation of interest. However, a significant error is introduced if the targeted EV subpopulation has a size, and thus hydrodynamic diffusion coefficient, that differs from the mean size and diffusion coefficient of the whole EV population and/or if the EVs become deformed upon binding to the surface. We here demonstrate a new approach to determine the mean size (or effective film thickness) of bound nanoparticles, in general, and EV subpopulation carrying a marker of interest, in particular. The method is based on operating surface plasmon resonance simultaneously at two wavelengths with different sensing depths and using the ratio of the corresponding responses to extract the particle size on the surface. By estimating in this way the degree of deformation of adsorbed EVs, we markedly improved their bulk concentration determination and showed that EVs carrying the exosomal marker CD63 correspond to not more than around 10% of the EV sample.

LA eng

DO 10.1021/acs.analchem.6b01860

LK http://dx.doi.org/10.1021/acs.analchem.6b01860

OL 30