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Food-induced changes of lipids in rat neuronal tissue visualized by ToF-SIMS imaging

Masoumeh Dowlatshahi Pour (Institutionen för kemi och kemiteknik, Analytisk kemi) ; Eva Jennische ; Stefan Lange ; Andrew G Ewing (Institutionen för kemi och kemiteknik, Analytisk kemi ; Institutionen för kemi och molekylärbiologi) ; Per Malmberg (Institutionen för kemi och kemiteknik, Analytisk kemi)
Scientific Reports (2045-2322). Vol. 6 (2016),
[Artikel, refereegranskad vetenskaplig]

Time of flight secondary ion mass spectrometry (ToF-SIMS) was used to image the lipid localization in brain tissue sections from rats fed specially processed cereals (SPC). An IonTof 5 instrument equipped with a Bi cluster ion gun was used to analyze the tissue sections. Data from 15 brain samples from control and cereal-fed rats were recorded and exported to principal components analysis (PCA). The data clearly show changes of certain lipids in the brain following cereal feeding. PCA score plots show a good separation in lipid distribution between the control and the SPC-fed group. The loadings plot reveal that the groups separated mainly due to changes in cholesterol, vitamin E and c18:2, c16:0 fatty acid distribution as well as some short chain monocarboxylic fatty acid compositions. These insights relate to the working mechanism of SPC as a dietary supplement. SPC is thought to activate antisecretory factor (AF), an endogenous protein with regulatory function for inflammation and fluid secretion. These data provide insights into lipid content in brain following SPC feeding and suggest a relation to activating AF.

Nyckelord: ion mass-spectrometry, antisecretory factor, alzheimers-disease, menieres-disease, cell-membranes, brain-tissue, cholesterol, phosphatidylethanolamine, localization, protein, Science & Technology - Other Topics


Article Number: 32797



Denna post skapades 2016-09-26. Senast ändrad 2017-06-16.
CPL Pubid: 242306

 

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Institutioner (Chalmers)

Institutionen för kemi och kemiteknik, Analytisk kemi
Institutionen för biomedicin (GU)
Institutionen för kemi och molekylärbiologi (GU)

Ämnesområden

Kemi
Biologiska vetenskaper

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